Primer

Part:BBa_K4213009:Design

Designed by: Ioannis Retalis   Group: iGEM22_Thessaly   (2022-08-27)


Reverse Primer for TetO7 sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Tms


Source

Designed with the help of SnapGene and NEB Tm Calculator

References